rat skin fibroblasts  (ATCC)

Journal: Frontiers in Public Health

Article Title: SIRT1 regulates dermal fibroblast senescence via impaired deacetylase function and mitochondrial dysfunction during skin aging induced by chronic oral cadmium exposure

doi: 10.3389/fpubh.2026.1779372

Figure Lengend Snippet: Exposure to CdCl 2 prompts an age-simulating phenotype in rat dermal fibroblasts. (A) Cell proliferation was monitored using a real-time cell analysis (RTCA) system following exposure to increasing concentrations of Cd (0, 0.2, 0.5, and 1 μM) for up to 48 h. (B) Western blot analysis of senescence-associated markers P53, P21CIP1, and P16INK4A. (C) SA-β-gal staining showing increased senescent cell burden. (D) Cell cycle distribution determined by flow cytometry, highlighting G1/G0 phase arrest. All experiments were performed 48 h post Cd exposure and were repeated independently three times (biological replicates). Independent experiments were performed in triplicate. Quantitative results are expressed as the mean ± SD. * p < 0.05, ** p < 0.01 vs. control group.

Article Snippet: The primary rat skin fibroblasts were derived from Pregnant Sprague–Dawley (SD) rats at 18 days gestation.

Techniques: Cell Analysis, Western Blot, Staining, Flow Cytometry, Control

Journal: Frontiers in Public Health

Article Title: SIRT1 regulates dermal fibroblast senescence via impaired deacetylase function and mitochondrial dysfunction during skin aging induced by chronic oral cadmium exposure

doi: 10.3389/fpubh.2026.1779372

Figure Lengend Snippet: Cd induces oxidative stress and DNA damage in rat dermal fibroblasts. (A) ROS accumulation assessed by DCFH-DA staining. (B) Total antioxidant capacity (AOC) measured via spectrophotometric assay. (C) Quantification of oxidative DNA damage via 8-OHdG levels. (D) Western blot analysis of DNA damage response proteins ATM and γH2AX. (E) TUNEL staining for apoptotic nuclear fragmentation. All experiments were performed 48 h post Cd exposure and were repeated independently three times (biological replicates). Independent experiments were performed in triplicate. Quantitative results are expressed as the mean ± SD. * p < 0.05, ** p < 0.01 vs. control group.

Article Snippet: The primary rat skin fibroblasts were derived from Pregnant Sprague–Dawley (SD) rats at 18 days gestation.

Techniques: Staining, Spectrophotometric Assay, Western Blot, TUNEL Assay, Control

Journal: Frontiers in Public Health

Article Title: SIRT1 regulates dermal fibroblast senescence via impaired deacetylase function and mitochondrial dysfunction during skin aging induced by chronic oral cadmium exposure

doi: 10.3389/fpubh.2026.1779372

Figure Lengend Snippet: Overexpressing SIRT1 in C3H lessens the aging effects caused by CdCl 2 exposure. (A) Western blot of P53, P21CIP1, and P16INK4A in fibroblasts with or without SIRT1 overexpression. (B) SA-β-gal staining of senescent cells. (C) Quantification of G1/G0 cell cycle arrest via flow cytometry. (D) Intracellular NAD + levels measured by ELISA. (E) TUNEL staining showing reduced apoptotic nuclei in SIRT1-overexpressing cells. All experiments were performed 48 h post Cd exposure and were repeated independently three times (biological replicates). Independent experiments were performed in triplicate. Quantitative results are expressed as the mean ± SD. * p < 0.05, ** p < 0.01 vs. control; # p < 0.05, ## p < 0.01 vs. Cd group.

Article Snippet: The primary rat skin fibroblasts were derived from Pregnant Sprague–Dawley (SD) rats at 18 days gestation.

Techniques: Western Blot, Over Expression, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay, TUNEL Assay, Control

Journal: Frontiers in Public Health

Article Title: SIRT1 regulates dermal fibroblast senescence via impaired deacetylase function and mitochondrial dysfunction during skin aging induced by chronic oral cadmium exposure

doi: 10.3389/fpubh.2026.1779372

Figure Lengend Snippet: SIRT1 restores redox balance, mitochondrial function, and genomic stability under Cd stress. (A) Intracellular ROS detected by DCFH-DA staining. (B) Measurement of total antioxidant capacity (AOC). (C) JC-10 staining reveals recovery of ΔΨm in SIRT1-overexpressing fibroblasts. (D) Western blot of γH2AX and ATM. (E) 8-OHdG levels as a marker of oxidative DNA damage. (F) Reversal of P53 and SOD2 acetylation upon SIRT1 overexpression. All experiments were performed 48 h post Cd exposure and were repeated independently three times (biological replicates). Independent experiments were performed in triplicate. Quantitative results are expressed as the mean ± SD. * p < 0.05, ** p < 0.01 vs. control; # p < 0.05, ## p < 0.01 vs. Cd group.

Article Snippet: The primary rat skin fibroblasts were derived from Pregnant Sprague–Dawley (SD) rats at 18 days gestation.

Techniques: Staining, Western Blot, Marker, Over Expression, Control